Newcastle disease virus antibody Rapid Assay
Catalog No. NDV20041117
Summary
This kit use NDV solid phase antigen to detect specific antibody against NDV antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and can be used dividually in multiple times
Reagents and contents
1 8× Coated microtiter strips: ready to use
2 1× concentrated Conjugate solution: ready to use
3 1× Washing solution: ready to use
4 1× Substrate A solution: ready to use
5 1× Substrate B solution: ready to use
6 1× Sample dilution: ready to use
7 1× Stopping solution: ready to use
Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor.
8 1× Positive control: ready to use
9 1× Negative control: ready to use
10 1× Instruction sheet
Test procedure
1 Diluting concentrated washing solution
Dilute the concentrated washing solution with distilled water or deionized water at 1:10, e.g. add 270 ml distilled water in 30 ml concentrated washing solution, and mix thoroughly.
2 Sample preparation:
Dilute the serum sample with sample dilution at 1:100, e.g. 5 μl serum + 495 μl sample dilution, mix thoroughly.
3 Adding samples and controls
Leave well A1 as reagent blank. Pipette controls and samples as follows:
100 μl of negative control and positive control respectively, and 100 μl diluted samples each into remaining wells.
Incubation at 37 ℃ for 30 minutes. Discard liquid of the wells and fill all wells with diluted washing solution, incubate for 1 minute and discard. Repeat washing procedure two more times as above. At the end of the washing step, carefully remove remaining fluid by tapping the strips on the tissue paper prior to the next step.
4 Adding conjugate solution
Add 1 drop conjugate solution into all wells and incubate at 37 ℃ for 30 minutes. Discard liquid of the wells and wash 3 times as described in step 3.
5 Adding substrates
Add one drop of substrate A solution and B solution respectively, incubate at 37 ℃ for 10 minutes.
Add one drop of stopping solution into all wells. Before reading results with ELISA microtiter plate reader, set the reagent blank well A1 to zero . Measure the absorbance of all wells at 450 nm (reference wavelength: 620 nm).
Results
Judgment by microtiter plate reader
Cut-off value = 2.1×A- (absorbance of negative control)
Interpretation of results
A450 (patient) ≥ cut-off: positive
A450 (patient) ＜ cut-off: negative
Notes
1 store at 2-8, make the temperature of the kit return to room temperature before use. screw down the cap after use, don't mix caps between diffirent bottles and don't mix components between diffirent kits
2 fill wells with distilled water when washing, 30 seconds each time, pour out the liquid. prohibit to use tap water or any other water.
3 recommand to judge results with instruments to improve comparability of testing.
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