Canine Distemper Virus (CDV) Antigen Rapid Diagnostic Card

Introduction
Canine distemper is widely spreading among various kinds of predators especially dogs through respiration system. Because of the difficulty to diagnose and treat this deadly disease promptly and effectively, the mortality of which is quite. According to related research, CDV particles can be detected in the sera of infected dogs about a week after infection. And two week later, some symptoms like fever, dry cough, conjunctivitis, vomit and diarrhea  may emerge early or late. In spite of some individual variance, 3-4 months old pups with low immunity are sensitive to CDV and can often occur serious CNS damage leading to death.
Based on fast immuno-chromatography technique, CDV Rapid Antigen Diagnostic Card can be utilized to detect canine distemper virus antigens simply and rapidly. When a sample derived from eye mucus, saliva, serum or plasma is added into sample-well on the card, it can move along membrane with underlying anti-CDV monoclonal antibody conjugated by colloid gold. If CDV antigens being present in samples, they can combine with the antibodies immobiled on test line and a wine-red line will appear in a few minutes. If no CDV antigens in samples, then color reaction won’t appear.

Contents
1 CDV diagnostic Card  10  pcs
2 Dropper              10  pcs
3 Swab              10  pcs
4 Dilution Buffer    1  bottle
5 Disposable gloves 5  pairs
6 Instruction        1  insert

Procedure
1） Tearing open an aluminum foil containing CDV Antigen Rapid Diagnostic Card.
2） Taking out the card.
3） Adding sample
  adding two drops of serum sample into dilution buffer. Mixing the buffer with the sample, then adding four drops of this mixture into sample-well on the card.
  As to eye mucus or saliva, collecting samples with swabs provided, then putting the swabs into dilution buffer. Mixing the buffer with the swab thoroughly and adding four drops of this mixture into sample-well on the card.
4） Placing the card on table for 10-15 minutes at room temperature.

Results
  Negative：no color reaction on Test Line (T Line),only on Control Line(C Line) being seen wine-red color reaction.

 Positive：Both on T Line and C line being seen wine-red color reaction.

Void：No color reaction on C Line.

Package
10 tests/kit

Shelf Life
12 months (Lot No. printed on aluminum foil

Storage
2-30 ℃

Notice
1） The card with broken foil should not be used.
2） This kit is only for diagnosis on CDV of dogs.
3） Avoid forming bublles while adding samples.
4） All cards and swabs used should be autoclaved or discarded according to local regulations.
5） The products out of date should not be used.

Q & A
1． What kinds of samples are suitable?
At early stage of infection, blood sampls are more sutable. Because at this stage no symptoms like dry cough, conjunctivitis, vomit and diarrhea appear, few viral particles exist in samples derived from eye mucus, saliva or nasal mocus. However, if some symptoms similar to canine distemper are present, it’s convenient and effective to cellect eye mucus and saliva samples for detection.  
2． When plasma samples used, why sometimes T Line can be seen color reaction with normal samples?
If anticoagulant such as EDTA is used to prepare plasma samples, false positive reaction will occur because of its disturbance on reaction. Please avoid using EDTA.
3． How much sample added into sample-well is suitable?
It’s  acceptable to add  50 – 150μl sample, although 100μl is ideal.
4． Will the samples collected from dogs inoculated with CDV vaccine appear positive reaction?
Because CDV vaccine contains live CDV, the virus can be present temporarily in body during immunization and positive result is possible accordingly.However, this kind of reaction may disappear 3-10 days later automatically.